Porous poly(L-lactic acid)(PLLA) scaffold membranes were prepared via. phase separation process.Chloroform, dichloromethane and 1,4-dioxane were used as solvent and, ethyl alcohol was used as non-solvent.Morphologies, mechanical properties and mass transfer characteristics of the scaffold membranes were investigated throughSEM, stress-strain test and glucose diffusion test. The scaffold membranes obtained from the casting solutions with chloroformand with dichloromethane showed similar morphologies. They showed sponge-like porous structure with the pore sizein the range of 3-10μm and, their porosities were in 50-80% range. Using 1,4-dioxane as solvent, nano-fibrous scaffoldmembranes with porosities over 80% were fabricated. When the polymer content in the solution with 1,4-dioxane was loweredto 4%, highly porous, macroporous and nano-fibrous scaffold membranes were obtained. The size of the macroporewas tens of the microns and the porosity was around 90%. These results indicate that the solvent has significant effect onthe scaffold membrane structure and, that scaffold membranes with various structures can be fabricated through phase separationmethod by choosing solvent and by controlling polymer concentration in the casting solution.